A new study published in Nature Communications has found that enzymes utilized by bacteria for breaking down mucus within the gut may provide a beneficial biomarker for the diagnosis of intestinal diseases.
Through the study, researchers from Newcastle University and the University of Birmingham, have discovered and characterized one of the crucial enzymes in the breakdown of mucus. They successfully demonstrated the manner in which the enzyme aids the bacteria in chemical decomposition and seek nourishment from the sugars in mucus layers lining the gut.
Potential to Develop New Diagnostics for Intestinal Diseases
The research offers a significant step forward in our understanding of the complex co-dependent relationships at work in the gut, about which little is currently known. Because the mechanism used by the enzyme is particularly distinctive, the researchers anticipate it can be used in the development of new diagnostics for intestinal diseases.
The molecules in mucus, called mucin, are constantly produced by the body to generate the layer of mucus in the gut that provides a barrier between the gut's complex populations of bacteria and the rest of the body. Mucin contains chains of sugar molecules called glycans, and these also provide an essential source of nutrients for bacteria.
The team investigated how this enzyme sits on the outside of the bacterial cell and clips away parts of the mucin molecule, taking them inside the bacterial cell to be consumed. Because glycans are known to change when certain diseases are present in the body, the researchers anticipate it will be possible to use the enzymes to take a snapshot of the glycans within a biopsy and use that as a biomarker for early detection of the disease.
Useful Biomarker for Studying Disease
Lead researcher, Dr. Lucy Crouch, of the University of Birmingham's School of Biosciences, explains: "Mucus is structured a bit like a tree, with lots of different branches and leaves. Lots of the enzymes discovered so far might clip away some of the leaves to eat, but the enzyme we studied will clip away a whole branch - that's quite a distinctive mechanism and it gives us a useful biomarker for studying disease."
The team has investigated this process in three different diseases. They examined tissue from adults suffering from ulcerative colitis and colorectal cancer, and from preterm infants with necrotizing enterocolitis, a serious illness in which the gut becomes inflamed and can start to die. They found that by adding the enzyme to the samples and labeling the glycans with a fluorescent dye, they were able to get useful information about the glycan structure.
Dr. Crouch adds: "Although we still don't fully understand what the glycan structures are made from and how these vary between different tissue types, we can see that the differences in structure between health and non-healthy tissue is quite distinctive. We hope to be able to use these enzymes to start producing better diagnostics for the very early stages of these diseases."
(With inputs from agencies)