Real Madrid forward Gareth Bale has been ruled out of Wales' next two World Cup qualifiers against Georgia and the Republic of Ireland with a calf strain.
The 28-year-old could be out for a month and that would also rule Bale out of the play-offs on 9-14 November, as reported by ESPN.
Bale joined the Wales squad on Monday and, despite sitting out training, it had been hoped he would be fit. But a scan revealed the severity of the injury and means Bale will not travel to Georgia for Friday's game in Tblisi and will play no part in the Cardiff clash with Republic of Ireland three days later, according to the BBC.
As had been earlier reported by the BBC, Real manager Zinedine Zidane had played down concerns about Bale's fitness, saying he rested him for Sunday's win over Espanyol as a precaution.
Wales are second in Group D, trailing leaders Serbia by four points with two matches remaining, and Barnsley striker Tom Bradshaw has been called up to the Wales squad in Bale's place.
Second place could be enough to earn Chris Coleman's team a place in the play-offs, for which Wales would realistically need at least four points from the games against Georgia and the Republic of Ireland.
Their chances of doing so will be harmed by the injury to Bale, who has scored four goals so far in this campaign. Wales have failed to win a match without the Real Madrid forward since 2013, drawing three and losing three matches in his absence.
The former Tottenham star has also been in fine form for Real, scoring a brilliant goal in last week's 3-1 Champions League win over Borussia Dortmund before being forced off with the muscular problem which has sidelined him for this month's qualifiers.
BBC pundit and ex-Wales international Robbie Savage said: "For Wales to be without Gareth Bale for two massive games is devastating. His passion and desire to play for Wales is there for all to see... it is a huge, huge blow.
"It will all be on the last game with Ireland, I believe. A point in Georgia won't be a bad result for me now. But even if Wales win their two games, that might not be enough. These are two of the biggest games in Welsh football history."