Signs that China is stepping in to steady the yuan after its recent sharp fall also helped ease investors' fears of a possible global currency war, though the yuan slipped further in morning trade, keeping markets guessing.
MSCI's broadest index of Asia-Pacific shares outside Japan was little changed in early trade but turned lower and was down 0.2 percent by 0459 GMT.
Japan's Nikkei .N225 slid 0.4 percent on worries that a stronger yen will put more pressure on its struggling exporters.
Still, selling pressure was mild compared with the last few sessions, when trade tensions intensified by the day.
MSCI Asia ex-Japan has tumbled 8.3 percent in its latest losing streak.
S&P e-mini futures ESc1 fell 0.5 percent. Wall Street had gained overnight and MSCI's broad gauge of stocks across the world rose for the first day in seven sessions.
Panicky selling of riskier assets eased after the People's Bank of China took steps to steady the yuan, with a firmer-than-expected mid-point fixing on Tuesday and state banks mopping up dollars, sources told Reuters.
Wednesday's yuan fixing was weaker, however, and just a hair away from the key 7 per dollar level, keeping markets nervous about China's intentions. Beijing let spot yuan break through the 7 level on Monday for the first time in 11 years, days after Washington threatened to impose more tariffs.
U.S. President Donald Trump dismissed fears of a protracted trade war on Tuesday despite a warning from Beijing that labeling it a currency manipulator would have severe consequences for the global financial order. Washington announced that move on Monday hours after the yuan tumbled.
While Trump played down the prospect that the trade dispute could be drawn out, St. Louis Federal Reserve Bank President James Bullard said the U.S. central bank may be stuck with a volatile global trade environment for years.
Comments from Larry Kudlow, director of the White House National Economic Council Kudlow, may have also tempered some jitters. He said on Tuesday the Trump administration wants to continue trade talks with China and is still planning to host a Chinese delegation for talks in September.
Many investors believe Trump cannot afford prolonged instability in financial markets since his reputation was staked so closely on economic growth and the success of the U.S. stock market.
The offshore yuan CNH=D3 was trading at 7.0812 per dollar, off a record low of 7.1382 on Tuesday but still down 0.39 percent on the day.
Onshore yuan CNY=CFXS opened at 7.0369 and was changing hands at 7.0458 at midday, down 0.3 percent.
For Beijing, further yuan weakness could "hurt as much as it helps", said Stefan Hofer, chief investment strategist at LGT Bank Asia in Hong Kong.
"It is counterproductive to depreciate to 8 or even 7.5 as it increases costs to importers and debt service costs much higher for Chinese issuers that borrow in dollars and have income streams in renminbi," he said.
Overall, market sentiment remained fragile.
With currencies thrown into the mix of the trade war, "investors with exposure to Asian equities are now faced with multiple factors, rather than straightforward earnings outlook," said Jim McCafferty, head of equity research for Asia ex-Japan at Nomura.
Goldman Sachs said it no longer expects a trade deal to be struck before the November 2020 U.S. presidential election, while Morgan Stanley warned that more tit-for-tat tariffs could tip the world economy into recession by the middle of next year.
That rather grim backdrop supported safe-haven assets, with gold hitting a six-year high of $1,489.76 per ounce on Wednesday. It last stood at $1,485.
U.S. bonds have also retained much of their gains made in the past week. The 10-year Treasuries notes yielded 1.66 percent percent US10YT=RR, lowest since 2016, as investors bet on another rate cut by the Federal Reserve in September.
In the currency market, the dollar traded at 106.10 yen JPY=, down 0.33 on the day but off Tuesday's seven-month low of 105.52.
The New Zealand dollar NZD=D4 slumped to $0.6378, a level not seen since 2016, after the central bank eased more aggressively than markets expected, cutting 50 basis points off its official cash rate, to counter pressure on the economy from global trade disputes.
Oil prices also weakened, with global benchmark Brent crude slipping to seven-month lows, as trade tensions between the U.S. and China intensified worries about weakening world demand.
Brent crude futures were down 0.15 percent to $58.85 a barrel, near its low on Tuesday of $58.55, a trough last seen in early January.